Response of Extracellular Polymeric Substance Composition in Chlorella pyrenoidosa to Acetaminophen Stress

The experiment set up three AAP concentration gradients of 0 (CK), 25 (L), and 75 mg · L ⁻¹ (H). During the 12 day culture period, samples were collected every 2 days to determine the content of polysaccharides (PS) and proteins (PN) in EPS; Samples were collected on days 0, 6, and 10, and the functional group structure and fluorescence component characteristics of EPS were determined using Fourier transform infrared spectroscopy (FTIR) and three-dimensional fluorescence spectroscopy (3D-EEM), respectively.

Furthermore, parallel factor analysis (PARAFAC) was used to mathematically analyze the fluorescence spectra. The dataset includes the following content: (1) time-series measurement data of polysaccharide and protein content and their ratio (PS/PN) in EPS of Chlorella vulgaris treated with different concentrations of AAP; (2) FTIR raw spectral data and characteristic peak assignment information of EPS samples; (3) Original fluorescence spectrum data of EPS samples.