Omics · study · 2026
Exploring DNMT1 genome binding under homeostatic and hypomethylating conditions [ChIP-seq]
Listed in NCBI GEO
Genome wide binding analysis of endogenous DNMT1 has yet to be succefully determined, owing to the difficulty of preserving DNA interactions during sonication.
Description
Using a light sonication protocol, we successfully ChIP DNMT1 and reveal its intrinsicDNA binding preferences genome wide under homeostatic and hypomethylating conditions. We discover that DNMT1 binds to accessible unmethylated CpG islands during homeostasis, then redistributes to hemi-methylated inaccessible DNA under hypomethylating conditions.
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K27me3 and endogenous DNMT1 in MV4-11 cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE233nnn/GSE233726 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE233726 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA977662 ncbi.nlm.nih.gov/bioproject/PRJNA977662 ↗
project · from NCBI GEO
- PubMed 42481757 pubmed.ncbi.nlm.nih.gov/42481757 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE233726 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |