Enhanced bioanalytical methods using molecularly imprinted polymers coupled to liquid chromatography-mass spectrometry

Additionally, the monitoring of pollutants that interact with biological systems is critical for establishing links between environmental exposure and disease development. However, existing analytical methodologies frequently exhibit deficiencies in sensitivity, selectivity, capturing efficiency, and reproducibility, particularly when applied to complex biological matrices.

This thesis addresses these challenges through the development of molecularly imprinted polymers (MIPs) for the selective extraction and quantification of thyroxine, bile acids, and perfluorocarboxylic acids (PFCAs) via liquid chromatography-mass spectrometry (LC-MS).</div><div><br></div><div>The application of MIPs offers a viable approach for achieving high selectivity in biomarker analysis; however, this methodology is not intended to replace immunoassays for thyroxine due to the limited accessibility of LC-MS in clinical settings.