<p>Model and experimental validation of acute and cyclic temperature responses in peripheral clocks.</p>

<p><b>A</b>: Model simulation of a 2-hour heat pulse (41°C, shaded gray) applied at CT4, showing <i>Per2</i> mRNA (solid line), <i>Per2</i> mRNA without heat pulse (dashed line), HSF1 trimers, and HSF1. The heat pulse at CT4 induces a marked acute increase in <i>Per2</i> mRNA compared to the no-pulse condition. <b>B</b>: Quantitative comparison between experimental values reported by Ohnishi et al. [<a href="ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1014831#pcbi.1014831.ref041" target="_blank">41</a>] for different peripheral tissues and the simulated fold-change in <i>Per2</i> mRNA expression following a 2-hour temperature elevation (41°C vs. 37°C). <b>C</b>: Entrainment of peripheral clock to opposing (inverted) temperature cycles.

The top shows simulated <i>Per2</i> oscillations synchronized to antiphase temperature cycles (36°C/38.5°C). The bottom shows an author-generated heatmap visualization of the experimental data reported by Buhr et al. [<a href="ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1014831#pcbi.1014831.ref021" target="_blank">21</a>]. <b>D</b>: Polar plot of the simulated <i>Per2</i> peak phases on days 2, 3, and 4 after entrainment under opposite temperature cycles, shown as three concentric circles from inner to outer.