<p>Significantly lower IgG-SC numbers in hospitalized patients compared to subclinical cases.</p>

Created in Biorender. (Right) Representative images of the DropMap bioassay including relocation onto the beadline in droplets of DENV1 sE-dimer (FITC), DENV1 sE (TRITC) and DENV2 sE-dimer (IR) in the absence (0 nM; negative control) or presence of anti-DENV1/DENV2 E protein mAb ADI24-294 (10nM; positive control). <b>B.</b> IgG secreting rate from (left) total IgG-SC or from (right) IgG-SC binding to sE and/or sE-dimer of DENV1 or DENV2 in PBMCs from hospitalized dengue patients and subclinical cases at day 5 post RT-qPCR confirmed DENV infection, corresponding to day 7-8 after onset of symptoms.

Horizontal bars represent median and IQR. Each dot represents one IgG-SC. <i>p</i> values were calculated using two-by-two contrast comparisons after linear modelling. ns, not significant<b>. C.</b> Proportion of IgG-SC not binding any DENV E protein (non-specific IgG-SC; white boxes), or binding at least one DENV1 E protein (to sE and/or sE-dimer; green boxes), or binding at least one DENV2 E protein (to sE and/or sE-dimer; blue boxes) in age-matched Cambodian healthy donors (HD) or in individuals infected with either (left) DENV1 or (right) DENV2.