Lipidomic profiles of human milk and yak milk

Subsequently, XCMS software was applied to refine the data through retention time correction, peak identification, extraction, integration, and alignment processes, applying a minimum threshold of 0.5 and a cutoff criterion of 0.6. For lipid identification, our laboratory's proprietary R package, built upon XCMS and integrated with the lipid blast database, was utilized.

Milk lipids and fatty acids (FAs) were quantified in units of μg/mL and g/100 total FAs, respectively. Statistical analysis of variations across different mammalian milk samples was conducted using Student's t-test, with statistical significance determined at an alpha level of 0.05, implying that a p-value less than 0.05 signifies a significant difference in the data.