The spatiotemporal dynamics of postnatal vascularization in the mouse brain

However, how vascularization progresses throughout the brain to integrate volumetric growth with neuronal circuit maturation is unclear. To address this spatiotemporally, we developed LAMBADA—a resource for registering and annotating optically cleared developing mouse brains with high temporal resolution, enriched by aligned spatial transcriptomics.

Using this resource, we identified three distinct, brain-wide phases of postnatal cerebral vascular development: (1) an isometric expansion phase, characterized by the expression of well-known transcriptomic signatures; (2) a regional specialization phase, which coincides with neuronal maturation and synaptogenesis; and (3) a refinement phase, marked by the stabilization of vascular networks and synapses. We delineate the molecular and structural mechanisms underlying these phases by correlating vascular remodeling with spatial transcriptomic gene expression.