ATAC-seq profiling of chromatin accessibility in Vigna radiata leaves under direct and pre‑conditioned salt stress

Chromatin accessibility plays a crucial role in transcriptional regulation under stress conditions. In this study, we investigated genome-wide chromatin accessibility dynamics in mung bean (Vigna radiata cv.

Weilv 9) leaves under salt stress using ATAC-seq. Two treatment groups were established: T1 (pre-conditioned with 100 mM NaCl for 24 h, recovered for 48 h, then re-exposed to 100 mM NaCl) and T2 (direct exposure to 100 mM NaCl without preconditioning). Seedlings were grown hydroponically in half-strength Murashige and Skoog medium under controlled environmental conditions.