Data · dataset · 2024
Mechanism of miR-16-5p alleviates airway inflammation in asthma rats by targeting the TXNIP/NLRP3 signaling axis
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Objective To investigate the mechanism of miR-16-5p alleviates ovalbumin (OVA)-induced airway inflammation in asthmatic rats by targeting thioredoxin-interacting protein (TXNIP)/NOD-like receptor thermoprotein structural domain-related protein 3 (NLRP3) signaling axis.
Description
Methods
Male Sprague-Dawley rats were randomly divided into the control, model, miR-16-5p mimic, NC mimic, miR-16-5p inhibitor, and NC inhibitor groups, with 6 rats in each group. A mixture of 10% OVA and 10% aluminum hydroxide was used for sensitization, and 4% OVA was given by nebulized inhalation to stimulate asthma. Dual luciferase reporter assay was performed to verify the target binding relationship; microscopic was used to observe inflammatory cell numbers in bronchoalveolar lavage fluid (BALF); ELISA was used to detect IL-1β, IL-6, TNF-α, and MCP-1 levels; H&E staining was used to observe morphological changes of lung tissue; Masson staining was used to observe bronchial collagen deposition; Immunohistochemical staining was used to detect TXNIP and NLRP3 expression; Western blot was used to detect TXNIP, NLRP3, ASC, cleaved-caspase-1, and IL-1β proteins expression.Results Luciferase reporter assay showed that miR-16-5p could target and regulate TXNIP.
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Compared with the control group, the model group rats had damaged lung tissues, thickened airway walls, and massive collagen deposition; an increased number of inflammatory cells, significantly higher expression levels of IL-1β, IL-6, TNF-α and MCP-1 (p < 0.05); TXNIP, NLRP3, ASC, cleaved-caspase-1, and IL-1β protein expression levels were significantly elevated in lung tissues (p < 0.05). Compared with the model group, lung histopathological damage was significantly improved in asthmatic rats after overexpression of miR-16-5b, and collagen deposition was reduced; the number of inflammatory cells was decreased; the levels of IL-1β, IL-6, TNF-α and MCP-1 were significantly reduced (p < 0.05); the levels of TXNIP, NLRP3, cleaved-caspase-1, and IL-1β protein expression levels were significantly reduced (p < 0.05).
MiR-16-5p inhibitor further aggravated the airway inflammatory response in asthmatic rats.Conclusion miR-16-5p can alleviate airway inflammation in asthmatic rats by targeting the TXNIP/NLRP3 signaling axis.
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Where it is published
- DOI doi.org/10.57760/sciencedb.j00217.01530 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.j00217.01530 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |