Omics · study · 2026
Transcriptomic Analysis of Caco-2 Cells Co-cultured with KERP2-related Entamoeba histolytica Strains
Listed in NCBI GEO
KERP2 is a secreted parasitic factor in Entamoeba histolytica that interacts with host colon epithelial cells, potentially contributing to pathogenesis.
Description
To investigate its influence on host gene expression, RNA-seq was performed on Caco-2 cells co-cultured with HA-KERP2-overexpressing E. histolytica (HA-KERP2), psAP-KERP2gs (partial KERP2 knockdown), or wild-type G3 strains, alongside untreated controls. Differential expression analysis identified upregulation of key pro-inflammatory mediators (IL1B, IL36G), signaling adapters (SLA), and heme-related genes (HRG) in HA-KERP2-treated cells, while cytoskeletal regulators (CYTIP, MYOCD), stress-response factor (NUPR1), and solute transporters (SLC15A3) were downregulated.
In contrast, psAP-KERP2gs-treated cells exhibited increased expression of extracellular matrix components (TNC), contractility regulators (MYL7), and metabolic enzymes (CYP4B1), while genes involved in cilia function (CFAP43), solute transport (SLC2A12), and the pentose phosphate pathway (TKTL1) were downregulated.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE290nnn/GSE290901 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE290901 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1230282 ncbi.nlm.nih.gov/bioproject/PRJNA1230282 ↗
project · from NCBI GEO
- PubMed 41888128 pubmed.ncbi.nlm.nih.gov/41888128 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE290901 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |