Replication data for: ATP synthase inhibition, an overlooked confounding factor in the mitochondrial stress test - statistical analysis supplement

This inhibition was recently shown to cause a glycolysis-dependent underestimation of two key mitochondrial respiration parameters, maximal respiration (MR) and spare respiratory capacity (SRC), in tumor cells. However, it is unknown if test substances (toxins, drugs, signaling molecules, etc.), especially those affecting glycolysis, can impact the underestimation of MR and SRC caused by CV inhibition and thereby produce potentially erroneous results.

The objective of the present study was to determine if the inhibition of CV in the mitochondrial stress test can act as a confounding factor when measuring MR and SRC in intact non-tumor cells exposed to exemplificatory test substances that affect energy metabolism: Ni2+ 56 and lipopolysaccharides (LPS). Murine bone marrow-derived macrophages were exposed to Ni2+ 57 (0–72 ppm) or LPS (1 µg/mL), and oxygen consumption rates were measured by extracellular flux analysis using the mitochondrial stress test, with and without CV inhibition.