<p>Effect of labeling and experimental system on the G_s protein mobility.</p>

<p>A) Median diffusion coefficients of G_s heterotrimers observed using overexpression of Gα_s and Gβ₁ under the CMV promoter, and underexpression of Gγ₂-HaloTag under the minP promoter (G_s: <i>n</i> = 15, observed range = 0.23–0.46 μm²/sec), as well as through overexpression of Gγ₂ and Gβ₁ under the CMV promoter, and underexpression of Gα_s-HaloTag under the minP promoter (G_sHaloTag; <i>n</i> = 15, observed range = 0.19–39 μm²/sec).

Additionally, diffusion coefficient medians of Gγ₂-labeled heterotrimers containing the Gα_s-ΔCt2 mutant, missing two C-terminal amino acids, are shown (G_s-ΔCt2; <i>n</i> = 10, observed range = 0.08–0.58 μm²/sec). B) Diffusion coefficient medians of the G_s protein (<i>n</i> = 15, observed range = 0.23–0.46 μm²/sec) coexpressed with the vasopressin 2 receptor (G_s + V2R, untreated; <i>n</i> = 11, observed range = 0.1–0.64 μm²/sec) and treated with arginine vasopressin 1μM (G_s + V2R+AVP, separated into time intervals post-treatment; 0–15 min: <i>n</i> = 4, observed range = 0.09–0.16 μm²/sec; 16–30 min: <i>n</i> = 5, observed range = 0.13–0.51 μm²/sec; 31–45 min: <i>n</i> = 6, observed range = 0.08–0.44 μm²/sec).