Omics · study · 2026
Transcriptional profiling of estrogen-responsive uterine genes in Rptorf/f Pgrcre/+ mice
Listed in NCBI GEO
To investigate the molecular mechanisms by which mTORC1 signaling inactivation contributes to thin endometrium, we performed uterine transcriptomic profiling in ovariectomized Rptorf/f Pgrcre/+ mice (n=3) and Rptorf/f control (n=3) mice 24 hours after estrogen stimulation.
Description
RNA was extracted with TRIzol, and quality-confirmed (A260/280 >1.8, RIN >8.0). Stranded mRNA libraries were prepared (TruSeq Stranded mRNA LT Kit) and sequenced on Illumina Novaseq 6000.
Reads were aligned to GRCm38.p6 (HISAT2), and gene expression was quantified as FPKM (Cufflinks) and read counts (HTSeq-count).
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312338 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312338 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1373111 ncbi.nlm.nih.gov/bioproject/PRJNA1373111 ↗
project · from NCBI GEO
- PubMed 41826809 pubmed.ncbi.nlm.nih.gov/41826809 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE312338 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |