Molecular Dynamics of Immortalized Chicken Fibroblasts from Adherent Cultivation to Carrier-Free Suspension Culture

Initially, we determined the optimal growth conditions necessary for suspension cell culture. The adherent DF-1 cells (2D) were then adapted to suspension culture, resulting in short-term (3D_E) and long-term (3D_L) suspension cells.

Growth metrics were monitored throughout the suspension phase. Subsequently, RNA-seq analysis was conducted to identify key genes and signaling pathways that exhibited distinct changes in 2D, 3D_E and 3D_L cells. The transcriptome data guided further investigations into the transdifferentiation of 2D, 3D_E, and 3D_L cells into lipids and their collagen secretion capabilities.