Data · dataset · 2026
Bioactivity analysis of a novel peptide, QUB-3368, identified from the skin secretion of the torrent frog, (Amolops hainanensis)
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32638221.v1
Antimicrobial peptides (AMPs) are a group of biological molecules with great potential as novel therapeutics for bacterial infections.
Description
AMPs are naturally occurring peptides that exhibit potent killing or inhibition of a broad range of microorganisms, including bacteria, fungi and viruses. In this study, a peptide, namely QUB-3368, was identified from the skin secretion of the Chinese torrent frog, Amolops hainanesis, and its bioactivity was evaluated by a series of bioassays.
The peptide encoding cDNA was initially cloned by “shotgun” cloning and the full length of the mature peptide sequence was obtained. Subsequently, the peptide was synthesised through solid-phase peptide synthesis (SPPS) and purified by reverse-phase high-performance liquid chromatography (HPLC). After that, the purified peptide was confirmed using a MALDI-TOF mass spectrometer.
Read the rest (1 more)
Three bioassays, including antimicrobial assay, MTT assay and haemolysis assay, were performed. It was found that peptide QUB-3368 showed significant inhibitory and killing effects against a gram-negative bacterium, E. coli (ATCC® CRM 8739TM) (MIC/MBC=2 µM), and a gram-positive bacterium S. aureus (ATCC® CRM 6538TM) (MIC/MBC=32 µM). However, the peptide displayed a weak activity against C. albicans (ATCC® CRM 10231TM) with an MIC of 256 µM. QUB-3368 also demonstrated a significant inhibitory effect on the proliferation of NCI-H838 non-small cell lung cancer cell line in a concentration-dependent manner with an IC50 of 25 μM. The peptide possessed a moderate haemolysis effect, and the HC50 value was 283.8 μM.<br><br><i>Thesis is embargoed until 31 December 2028</i>.
Links
Where it is published
- DOI doi.org/10.17034/32638221.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32638221 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32638221 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32638221 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |