Data · dataset · 2026
The first environmental DNA-based inventory of soil fungal diversity in Ukraine - ITS-based occurrences
Listed in GBIF
This dataset presents the first national-scale, DNA metabarcoding-based record of soil fungal communities in Ukraine, compiled following FAIR data principles.
Description
It is derived from 225 topsoil samples collected across accessible regions of Ukraine (June–November 2023), spanning five ecoregions including Carpathian montane forests, mixed forests, forest-steppe, and Pontic steppe habitats. Soil DNA was amplified using universal fungal-specific primers (ITS3/ITS4) targeting the internal transcribed spacer (ITS2) rRNA gene region, sequenced on an Illumina NextSeq 2000 platform, and resolved into Operational Taxonomic Units (OTUs) using QIIME2.
We assigned taxonomy folowing two-step approach. First, we automatically assigned taxonomy using scikit-learn Naїve Bayes classifier with RESCRIPt tool tained against UNITE reference dataset (v10). Then, to control quality of taxonomic accuracy, 3366 OTU was manually checked by six taxonomic experts by manually pairwise alignement against reference databases using Mycobank and NCBI Nucleotide BLAST web tools.
Read the rest (2 more)
Occurrence records are provided for 14,029 OTUs belonging to Kingdom Fungi, alongside its reference sequences. This dataset extends the area of Europe densely covered by soil eDNA sampling more than 800 km eastward and represents the first unified, national-scale eDNA characterization of soil fungal diversity in Ukraine. By adhering to contemporary biodiversity data standards, it supports advanced spatial analyses, diversity hotspot identification, and evidence-based planning for post-war ecological restoration.
The structured, standards-compliant format is intended to facilitate reuse by a broad range of stakeholders — including local communities, protected area managers, environmental agencies and NGOs, and researchers — regardless of their prior expertise with soil microbiome data.[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]
Links
Get the data
- Darwin Core Archive ukraine.ipt.gbif.no/archive.do?r=soil_fungi_ukraine_its ↗
download · from gbif
Where it is published
- GBIF dataset page gbif.org/dataset/341e5878-1075-40ac-bc52-b0fd7f693cb7 ↗
landing page · from gbif
- DOI doi.org/10.15468/rb47qz ↗
DOI / persistent id · from gbif
Catalogue records · 1
- GBIF API api.gbif.org/v1/dataset/341e5878-1075-40ac-bc52-b0fd7f693cb7 ↗
metadata API · from gbif
Topics
- From keywords
- Life Sciences
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| GBIF | 341e5878-1075-40ac-bc52-b0fd7f693cb7 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · gbif | connector:gbif@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · gbif | connector:gbif@1.0.0 | |
| created_date | source · gbif | connector:gbif@1.0.0 | |
| description | source · gbif | connector:gbif@1.0.0 | /description |
| license | source · gbif | connector:gbif@1.0.0 | |
| publication_date | source · gbif | connector:gbif@1.0.0 | |
| spatial | source · gbif | connector:gbif@1.0.0 | |
| temporal | source · gbif | connector:gbif@1.0.0 | |
| title | source · gbif | connector:gbif@1.0.0 | /title |
| updated_date | source · gbif | connector:gbif@1.0.0 |