Chlorophyll a data collected during the SIPEX II voyage of the Aurora Australis, 2012

Sea ice brine microalgae was collected from sackholes. Replicate samples were incubated in ambient air (~0.04% CO2), 0.1% CO2, 1.0% CO2 and 2.0% CO2 concentrations.

AT the end of the incubations the 50 ml samples were filtered through a 25 mm GF/F filter using vacuum filtration. The filters were placed in 15 ml plastic falcon tubes containing 10 ml of methanol, covered in aluminium foil and kept in the dark at 4 degrees C for 12 hours. Chl a concentration was measured using a 10AU Turner fluorometer following the acidification method of Strickland and Parsons (1972).