Construction of Silver Nanoparticle-based Artificial Hydrolases via Conformational Engineering and Study of Its Catalytic Mechanism

Using silver nanoparticles (AgNPs) as the scaffold, the study successfully mimicked the catalytic center of α-chymotrypsin—including the catalytic triad, oxyanion hole, and substrate-binding site—through peptide design and a three-step conformational control method (induction of α-helical conformation by trifluoroethanol, stabilization of the conformation via Ag–S bonds, and removal of the inducer by freeze-drying).

The dataset primarily includes the following content:Circular Dichroism (CD) data: Used to demonstrate the formation and maintenance of the α-helical conformation of the peptides on the AgNP surface, thereby validating the successful implementation of conformational engineering.Stopped-flow kinetics data: Including comparisons of catalytic activity before and after mutation of key residues, used to demonstrate the functional necessity of the catalytic triad, oxyanion hole, and substrate-binding site, as well as their synergistic effects.Catalytic activity data: The turnover number per active site measured using p-nitrophenyl acetate (p-NPA) as the substrate.Substrate scope expansion data: Activity data showing the catalytic degradation of diethyl phthalate (DEP), an environmental priority pollutant, by Silverzyme, demonstrating its ability to hydrolyze non-activated esters.