Rodrigues et al. Fluctuating heat stress data

Files available: We provide 2 txt files: FLUCT_mating_fertility; FLUCT_productivity_sexratio. FLUCT_mating_fertility: This file contains the raw data of male mating propensity and fertility under four thermal conditions. Table headers are explained below: LineStatus - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. (2020)*.

(High – lines whose fertility did not decline as temperature increased; Low – lines whose fertility declined substantially as temperature increased) DGRP- line identity Wolbachia- line infection status (y – infected; n- uninfected) Haplotype- haplotypes identified in Bevers et al. (2019)* Inv1/2/7/8/9 - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion.

ST/INV = unfixed status in the line) Temperature - mean developmental temperature experienced (25°C or 29°C) ThermalRegime - thermal variation experienced (Const - constant or Fluct - fluctuating regime) Vial – vial where each pair was kept EggCollDay – day the eggs were collected and placed in one of the thermal conditions. PairingDay – day males and females were paired Mating – mating propensity (1, if mating observed; 0, if no mating observed) Larvae – fertility (1, if larvae present; 0, if no larvae present) FLUCT_productivity_sexratio: This file contains the raw data of male productivity and sex ratio under four thermal conditions.

Table headers are explained below: LineStatus - classification based on the reproductive performance across three temperatures tested in Zwoinska et al. 2020. (High - whose fertility did not decline as temperature increased; Low - whose fertility declined substantially as temperature increased) DGRP- line identity Wolbachia- line infection status (y – infected; n- uninfected) Haplotype- haplotypes identified in Bevers et al. (2019)* Inv1/2/7/8/9 - the inversion status of each line for each of the main inversions characterized in the DGRP (ST = standard, INV = inversion.

ST/INV = unfixed status in the line) Temperature - mean developmental temperature experienced (25°C or 29°C) ThermalRegime - thermal variation experienced (Const - constant or Fluct - fluctuating regime) Vial – vial where each pair was kept EggCollDay – day the eggs were collected and placed in one of the thermal conditions. PairingDay – day males and females were paired Productivity – number of adult offspring produced.

Daughters – number of female offspring produced. Sons – number of male offspring produced. IGV – absolute deviation from the median as a measure of intra-genotypic variability. *Bevers, R. P. J., et al (2019).

Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila Genetic Reference Panel. Nature Metabolism, 1, 1226–1242. doi.org/10.1038/s42255-019-0147-3 Zwoinska, M. K., Rodrigues, L. R., Slate, J., & Snook, R. R. (2020). Phenotypic responses to and genetic architecture of sterility in response to sub-lethal temperature during development.

Frontiers in Genetics, 11, 573. doi.org/10.3389/fgene.2020.00573