Data · dataset · 2026
Quercetin alleviates Der p 2-induced inflammatory response in airway epithelial cells by inhibiting mitochondrial overactivation
Listed in ScienceDB
Objective To investigate the effects of the house dust mite allergen Der p 2 on mitochondrial function and inflammatory response in human bronchial epithelial cells (BEAS-2B) and to elucidate the interventional role of quercetin.
Description
Methods
Using BEAS-2B cells as the model, different concentrations of Der p 2 (0, 10, 20, 30 μg/mL) and quercetin (0, 5, 10, 20, 30, 40 μM) were treated alone or in combination. Cell viability was assessed using the CCK-8 method, the mRNA expression levels of epithelial-derived alarmins (TSLP, IL-33, IL-25) and pro-inflammatory cytokines (IL-4, IL-6, IL-8) were detected by qRT-PCR, the intracellular ATP levels were measured using a bioluminescence method, and mitochondrial membrane potential was detected by the TMRE fluorescent probe.Results Der p 2 significantly reduced BEAS-2B cell viability in a dose-dependent manner (P = 0.000 9), while quercetin alone had no significant effect.
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However, quercetin dose-dependently reversed the reduction in cell viability induced by Der p 2 (P < 0.000 1). Der p 2 significantly upregulated the mRNA expression of TSLP, IL-33, IL-25, IL-4, IL-6, and IL-8 in BEAS-2B cells (all P < 0.05). Quercetin inhibited the expression of these factors (all P < 0.001), except for IL-4.
Der p 2 exposure induced alterations in mitochondrial function in BEAS-2B cells: ATP production and mitochondrial membrane potential were significantly increased at 12 h (both P < 0.001), followed by a gradual decline. Quercetin significantly inhibited these effects (both P < 0.000 1).Conclusion Der p 2 mediates airway epithelial cell inflammatory response and reduced cell viability by inducing early mitochondrial hyperfunction and subsequent metabolic exhaustion.
Quercetin alleviates Der p 2-induced epithelial cell damage and inflammation by modulating mitochondrial energy metabolism. This study provides new experimental evidence for targeting mitochondrial metabolism in the intervention of allergic asthma.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.j00217.12496 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.j00217.12496 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |