Ikaros affects multiple lineages in hematopoietic development [RNA-Seq]

Here, we use the newly generated Ik-ZnF1-/- and Ik-ZnF4-/- mutant mice to study hematopoietic development in the BM. As an initial approach to study early hematopoietic development as well as non-lymphoid lineages, RNA-Seq was performed on "Hardy Fraction A" cells, without lineage depletion (B220+IgM-CD43+CD24-BP-1-), of wt and Ik-ZnF1-/- mice (two biological replicates of each).

Ik-ZnF4-/- mice have extremely few cells in the "Hardy fraction A" gate, and only one RNA-Seq experiment was performed on this cell population, with strongly reduced reads/genome coverage.