Omics · study · 2026
Lipofected mRNAs are spliced in Human cells
Listed in NCBI GEO
Current strategies for therapeutic use of mRNAs and for testing the function of specific mRNA isoforms assumes that transfected mRNAs are not further processed by cellular splicing machinery.
Description
Here we show that lipofected mRNAs can be spliced in human cells, utilizing canonical splice donor and acceptor sites and this is suppressed by spliceosome inhibition. This post-transcriptional splicing has important strategic implications for the use of transfected mRNAs in experiments and therapies.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341263 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341263 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1500039 ncbi.nlm.nih.gov/bioproject/PRJNA1500039 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE341263 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |