Data · dataset · 2026
The identification, bioactive evaluation and exploration of the active sites of antimicrobial peptides from the skin secretion of Odorrana margaretae
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32641299.v1
Antimicrobial resistance continues to escalate globally, underscoring the urgent need for novel therapeutic strategies.
Description
Among them, AMPs derived from amphibian skin secretions have attracted significant interest due to their potent and broad-spectrum activity. However, a fundamental challenge persists, the poor understanding of AMP active sites, particularly in non-model species such as Odorrana margaretae.
To address this gap, this study employed a multimodal strategy integrating gene cloning, SPPS, RP-HPLC/MALDI-TOF identification, and a series of functional and structural assays to characterise two AMPs: QUB1971 and Palustrin-OM. In Chapter 3, by sequence alignment analysis, Ile13 was identified as a critical residue in QUB1971, essential for antimicrobial activity. Its substitution with valine, analogue 13V, led to a complete loss of function, with MICs exceeding 512 μM across all tested strains.
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Analogue L, rationally designed based on structural insights, exhibited the best therapeutic index (TI = 8.7) among all derivatives. In Chapter 4, Alanine-scanning mutagenesis further identified Ile6, Lys11, Ile13 and Val14 as functional “hotspot” residues, where substitution led to a marked reduction with MICs exceeding 64 μM across all tested strains. In contrast, modifications at other positions were either neutral or enhanced activity, demonstrating a context-dependent role of individual residues and offering guidance for rational design strategies.
In Chapter 5, three truncated analogues of palustrin-OM were designed using the trypsin-cleavage mimicking strategy. Functional screening revealed that truncated peptide 2 not only retained but enhanced antimicrobial efficacy, as indicated by a lower overall GM (4.6 μM vs. 8.5 μM for Palustrin-OM), while markedly improving safety with a higher therapeutic index (TI overall = 6.9 vs. 1.0), thus presenting a promising candidate for further development.
Together, these complementary approaches established a robust framework for identifying active sites in antimicrobial peptides, thereby advancing the rational design of next-generation peptide therapeutics with improved potency and selectivity.<br><br><i>Thesis is embargoed until 31 July 2030</i>
Links
Where it is published
- DOI doi.org/10.17034/32641299.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32641299 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32641299 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32641299 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |