A novel peptide QUB-2562, discovered from the skin secretion of the frog, Agalychnis annae

During the last few decades, skin secretions of amphibians, especially frogs, were found to be a vast resource of antibiotic agents.<br><br>In this project, a novel peptide QUB-2562 derived from the skin secretion of Agalychnis annae was identified by molecular cloning.

Solid-phase synthesis was applied to synthesise the novel peptide. After the synthesis, High Performance Liquid Chromatography (HPLC) and Matrix-assisted laser desorption ionisation, time-of-flight (MALDI-TOF) mass spectrometry was used to identify and purify peptide QUB-2562. Then QUB-2562 was tested in several biological experiments and found to inhibit the growth of S. aureus (MIC = 2 µM; MBC = 2 µM), E. coli (MIC = 4 µM; MBC = 4 µM), and C. albicans (MIC = 8 µM; MBC = 16 µM).