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Data · dataset · 2026

<p>Complexome profiles of the F<sub>1</sub>F<sub>o</sub> ATP synthase complex.</p>

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<p>(A) Heatmap of the complexome profile from mitochondrially-enriched material of ATP synthase.

Description

Each row represents a gel slice, with the gel slice number indicated on the left and the molecular weight, based on the migration of mammalian mitochondrial complexes (black) and soluble proteins (blue), on the right. Subunit IDs are shown at the bottom of their corresponding profile.

Dark blue indicates the highest relative abundance (1) and white the lowest (0). Coomassie-stained Native PAGE gel on which the analysis was performed is depicted on the left of the profile. Data shown is from replicate 1.

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(B) Coulson plots depicting the composition of ATP synthase in key species from myzozoans and ciliates. <i>Perkinsus</i> subunits were used for homology searches using BLAST and jackhmmer. <i>T.t Tetrahymena thermophila</i>; <i>P.t Paramecium tetraurelia</i>; <i>T.g Toxoplasma gondii</i>; <i>P.f Plasmodium falciparum</i>; <i>C.p Cryptosporidium parvum</i>; <i>C.m Cryptosporidium muris</i>; <i>C.v Chromera velia</i>; <i>V.b Vitrella brassicaformis</i>; <i>P.g Polarella glacialis</i>; <i>S.m Symbiodinium microadriaticum</i>.

Full dataset in <a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.s011" target="_blank">S6 Table</a>. (C) Structure of ATP Synthase dimer from <i>Toxoplasma gondii</i> (PDB 6TMK, [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref017" target="_blank">17</a>]) with subunits conserved in <i>Perkinsus</i> coloured blue, and subunits not found coloured in pink.

Visualised using ChimeraX [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref098" target="_blank">98</a>].</p>

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