Data · dataset · 2026
Identification and evaluation of a bioactive peptide, QUB-3195, from the skin secretion of <i>Phyllomedusa sauvagei</i>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32640195.v1
Antimicrobial resistance has emerged as a greater hazard to public health than other issues throughout the past several years and antimicrobial peptides have been identified as viable therapeutics against drug-resistant bacteria due to their broad-spectrum action and low propensity to induce drug resistance.
Description
Peptides secreted from amphibian skins have a great range of bioactive functions and thus there has been a growing interest in peptides as clinical therapeutic agents.
The Dermaseptin peptides are a superfamily of antimicrobial peptides. They have a variety of low-cytotoxic antimicrobial and anticancer properties.In this study, QUB-3195, a novel Dermaseptin peptide, was isolated from the skin secretion of Phyllomedusa sauvagei. A cDNA library was constructed by using the isolation mRNA from frog skin secretion and ‘shotgun’ cloning, through the use of which the mature peptide sequence was deduced.
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The purified peptide was obtained by reversed-phase high performance liquid chromatography. In this research, QUB-3195 was found to exhibit a wide range of antimicrobial activity against tested Gram-negative bacteria, Gram-positive bacteria, and yeast. The MIC and MBC values for both tested bacteria were determined to be 1 μM.For the yeast strain, the MIC and MBC were both 16 μM. In addition, QUB-3195 inhibited a human lung cancer cell line (H838).
The half minimal inhibitory concentration (IC50, µM) of QUB-3195 was 39.9 µM. However, the peptide showed a weak inhibitory function of haemolysis activity with a HC50 value of 287.7 µM. In summary, due to its strong antibacterial and anticancer properties, QUB-3195 is a good option for developing novel medications for use in clinical settings. Also, with further modifications and experiments, QUB-3195 may be found to have better antimicrobial and anticancer functions in future studies.<br><br><i>Thesis is embargoed until 31st December 2029</i>.<br><br>
Links
Where it is published
- DOI doi.org/10.17034/32640195.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32640195 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32640195 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32640195 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |