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Omics · study · 2026

SNHG29 knockdown by CRISPRi and shRNA in M07e acute myeloid leukemia cells

Listed in NCBI GEO

The long non-coding RNA SNHG29 has been identified as a candidate essential gene in acute myeloid leukemia (AML) through a genome-wide CRISPRi depletion screen.

Description

To characterize the transcriptomic consequences of SNHG29 loss, we performed RNA-seq in M07e AML cells following SNHG29 knockdown using two independent methods: CRISPRi (two sgRNAs targeting the SNHG29 promoter, with Luciferase-targeting sgRNA as control) and shRNA (two shRNAs targeting SNHG29, with scrambled shRNA as control).

Three biological replicates were generated per condition. Differential expression analysis was performed using DESeq2.

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Expression profiling by high throughput sequencing · Homo sapiens
From keywords
Life Sciences
Inferred from text
RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
SourceKeyLast seenRaw
NCBI GEOGSE32677415 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencingsource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[modality].local:modality:rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (65%)
concepts[organism].NCBITaxon:9606source · NCBI GEOconnector:ncbi_geo@1.0.0/taxon
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title