The effects of IL-18 on the expression levels of IL-18Rα and CD63 in peritoneal lavage fluid macrophage subsets of sepsis mice

PLF IL-1β and IL-10 were measured by liquid chip technology, and the Pearson correlation analysis was performed between IL-1β and the ratios of M1 and IL-18Rα+ M1, and between IL-10 and M2, CD63+ M2 and IL-18Rα+ M2.Results It was observed in sepsis and IL-18-pretreated mice that the size of spleen white pulp and inflammatory cell infiltration around the splenic envelope increased. Expression of CD63 and IL-18Rα on M1 and M2 macrophages was elevated.

PLF IL-1β and IL-10 levels increased, with IL-1β highly positively correlated with the ratios of IL-18Rα+ M1, and IL-10 highly positively correlated with the ratios of CD63+ M2 and IL-18Rα+ M2..Conclusion Briefly, increased expressions of IL-18Rα and CD63 on M1 and M2 of sepsis and IL-18-pretreated mice, and the strong positive correlations between elevated PLF IL-1β and IL-10 levels with the ratios of IL-18Rα+ M1 and IL-18Rα+ M2, suggest that IL-18 may play an important role in the dysregulated inflammation response of sepsis by inducing M1 and M2 activation through IL-18Rα.