List of predicted secreted proteins of M. enterolobii (genome V1, INRA/JKI)

Use SignalP 4.0 that better discriminate between SP and N-terminal TM Put the -m 'file' option to produce a FASTA with cleaved SP signalp -f short -m MentV1-cleaved.fa -t euk MentV1.pep.fa > MentV1.SP --> there are 6,065 proteins with a predicted SP. 2. Use TMHMM-2.0 to predict TMs on the cleaved FASTA sequences. tmhmm --short MentV1-cleaved.fa > MentV1-cleaved-TM-short.txt --> there are 1,092 proteins with a SP and at least one TM (that is not included within the SP).

Thus 4,973 proteins with SP and no TM. 3. Generate a list of sequences with predicted SP an no predicted TM The accession numbers for the 4,973 proteins with SP and no TM is saved in MentV1.SP-no-TM.acc