Data · dataset · 2025
RT-qPCR
Listed in ScienceDB
RNA extraction and RT-qPCRTotal RNAs in the cell samples were extract using Trizol reagent. 1μg of RNA was reverse transcribed to cDNA with a StarScript II RT Mix with gDNA Remover kit.
Description
RT-qPCR was conducted with 1 μL of cDNA as the template using 2 × RealStar Power SYBR qPCR Mix according to the manufacturer's instructions. The GAPDH gene was used as the internal control.
The results were calculated with the comparative CT (2−ΔΔCT) method.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.28662 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Computed tomography 50%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.28662 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:ct | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (50%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |