<p>Cluster validations for adult glial annotations.</p>

(E) <i>rost</i>-<i>lacZ</i> reporter (cyan) is expressed only in subperineurial glia. <b>(F)</b> GFP-tagged <i>CG3036</i> (green) is expressed only in chalice glia. Vvl antibody (cyan) labels distal and proximal satellite glia. Optix (yellow) is present in chalice, marginal, epithelial, and proximal satellite glia.

(G) <i>b</i>-Gal4 (cyan) is expressed in distal and proximal satellite glia and in epithelial glia, but not in marginal. <b>(H)</b> <i>GstT4</i>-Gal4 (cyan) is expressed only in marginal glia. <b>(I)</b> <i>DAT</i>-Gal4 (cyan) is expressed in outer chiasm glia but only seen rarely in inner chiasm glia. <b>(J)</b> GFP-tagged <i>CG31663</i> (cyan) is expressed in proximal satellite glia and some medulla neuropil glia. This might reflect ensheathing- (mng+) <i>vs.</i> astrocyte-like (mng−) identity.

It is also expressed in outer and inner chiasm glia and in cortex glia. (K) <i>ome</i>-Gal4 (cyan) is expressed in inner chiasm glia, marginal glia and some medulla neuropil glia, which could reflect ensheathing (mng+) vs. astrocytes-like (mng−) identity. (L) <i>nkd</i>-<i>lacZ</i> reporter (cyan) is expressed in medulla neuropil glia but not in lobula neuropil glia. fg = fenestrated glia, pg = perineurial glia, spg = subperineurial glia, dsg = distal satellite glia, psg = proximal satellite glia, chg = chalice glia, eg = epithelial glia, mg = marginal glia, xgo = outer chiasm glia, cxg = cortex glia, lng = lobula neuropil glia, mng = medulla neuropil glia.

Gal4-UAS (<i>>></i>), <i>GFP/LacZ</i> from construct (<i>::</i>). Scale bar = 20 µm. The code used to generate the graphs can be found at <a href="zenodo.org/records/21890456" target="_blank">zenodo.org/records/21890456</a>.</p>