Data Sheet 1_Transcriptomic insights into the molecular regulatory mechanism of staminody in Chinese narcissus (Narcissus tazetta subsp. chinensis).docx

Bimolecular fluorescence complementation, luciferase complementation imaging, and co-immunoprecipitation assays demonstrated that NtTGA10 specifically interacts in vivo with the C-class MADS-box protein NtMADS6 (AGAMOUS), suggesting formation of a transcriptional complex. RT-qPCR analysis showed high expression of NtTGA10 in normal stamens but significant downregulation in petaloid stamens and other floral organs. This expression pattern implies that NtTGA10 not only participates in normal stamen development but also its downregulation may be closely associated with petaloid conversion.

Collectively, our findings suggest that the NtTGA10–NtMADS6 transcriptional complex serves as a critical regulator in maintaining stamen identity and repressing petaloid conversion. This study provides the first evidence for TGA–MADS box interplay in regulating floral organ identity in narcissus, offering novel insights into the molecular basis of floral organ plasticity and identifying potential targets for molecular design breeding in horticultural plants.</p>