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Data · dataset · 2026

Genome annotation files of Heterobiantes geniculatus

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This dataset presents the genome annotation of Heterobiantes geniculatus, encompassing repetitive sequences, non-coding RNAs, protein-coding genes, and functional annotations.

Description

The dataset cover image is credited to Bosco Yuen.Repeat annotation was performed by constructing a species-specific repeat library using RepeatModeler v2.0.7 (with LTR structural detection enabled), followed by genome-wide identification and classification of repeat elements using RepeatMasker v4.2.2.

Based on the alignment results generated by RepeatMasker, the Kimura 2-Parameter divergence for each transposable element (TE) family was calculated using the calcDivergenceFromAlign.pl script to evaluate evolutionary dynamics.Non-coding RNA annotation was conducted using Infernal v1.1.5 against the Rfam database to identify rRNAs, snRNAs, and miRNAs. tRNAs were predicted using tRNAscan-SE v2.0.12, and high-confidence candidates were filtered using the EukHighConfidenceFilter script.Protein-coding gene annotation was based on the MAKER v3.01.04 pipeline, which integrated multiple lines of evidence.

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First, transcriptome data was assembled using StringTie v3.0.1 to generate transcript evidence. Second, ab initio and homology-based predictions were performed using BRAKER v3.0.6 (incorporating Augustus and GeneMark-ETP) and GeMoMa v1.9. Finally, protein-coding gene structures were generated by integrating transcript, homologous protein, and ab initio prediction results.Functional annotation was achieved by aligning sequences against the UniProtKB v202503 database using DIAMOND v2.1.7.161 (high-sensitivity mode) to obtain sequence similarity information.

Conserved protein domains were identified using InterPro v5.70-102.0 (Pfam database), and GO classification and KEGG pathway annotations were completed using eggNOG-mapper v2.1.12 (eggNOG v5.0.2 database).

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