Omics · study · 2026
tRNA m1A modification in macrophages promotes translation of interferon pathway genes to enhance CD8+ T cell-mediated anti-tumor immunity [CAR-iMACs RNA-seq]
Listed in NCBI GEO
The involvement of tRNA m1A modification in the anti-tumoral tumor-associated macrophages (TAMs) has been validated in our study that macrophage-specific knockout of m1A “writer” enzymes TRMT61A leads to increased infiltration of TAMs, impaired CD8+ T cell function, and enhanced tumor growth.
Description
Since we observed that tumor-derived components suppresses tRNA m1A modification in macrophages, we sought to identify which component in the TME induces the down-regulation of tRNA m1A modification.
Here, we performed bulk RNA-seq using TRMT61AOE-CAR-iMACs and CAR-iMACs to ensure the effect of human Trmt61a overexpression on CAR-iMACs. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that signal transduction is the most up-regulated pathway in CAR-iMACs after human Trmt61a overexpression.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE288nnn/GSE288142 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE288142 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1216274 ncbi.nlm.nih.gov/bioproject/PRJNA1216274 ↗
project · from NCBI GEO
- PubMed 41535541 pubmed.ncbi.nlm.nih.gov/41535541 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE288142 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |