Oligosaccharides from Dendrobium officinale Ameliorate Metabolic Dysfunction in db/db Mice by Modulating Gut Microbiota, Short-Chain Fatty Acid Production, and Intestinal Barrier Integrity

However, the effects of oligosaccharides derived from Dendrobium officinale (DOO1) in T2DM remain unclear. This study evaluated the effects of DOO1 on metabolic parameters, gut microbiota, short-chain fatty acids (SCFAs) production, intestinal barrier function, and host metabolism in <i>db/db</i> mice.

DOO1 significantly improved glucose and lipid metabolic disorders and reduced pancreatic and hepatic injury. These effects were accompanied by changes in gut microbiota composition, characterized by increased abundances of Duncaniella, Muribaculum, and Paramuribaculum, along with elevated fecal SCFA levels. DOO1 improved intestinal barrier function, as indicated by reduced intestinal permeability and inflammatory markers (TNF-α, IL-1β, LPS), and increased expression of <i>Muc2</i>, ZO-1, and Claudin-1.