Benchmarking cell-type deconvolution in cross-platform transcriptomic data [Visium]

Results: We systematically benchmarked deconvolution performance using real-world cross-platform datasets and simulated data modeling distinct technological features. SpatialDecon and cell2location demonstrated the most reliable and consistent performance across both simulated and experimental settings across a broad range of technological biases. Conclusions: Our results highlight how the different deconvolution tools are affected by data properties that depend on technological differences between transcriptomic platforms.

Moreover, we provide practical guidelines for selecting computational methods dependent on experimental design for robust deconvolution of cross-platform transcriptomic data.