<p>Common genes expressed in early neuropil glia.</p>

<p><b>(A–F)</b> UMAPs representing the integration of all glial cells from L3, P15, P30, P50, P70, and adult stages. Panels show the expression levels of genes that are common to the initial trajectory of neuropil glia lineages. Genes shown were the first six genes selected from <a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3004015#pbio.3004015.s012" target="_blank">S4 Table</a> by highest avg_log2FC and pct.2 < 0.1 (cells from other clusters that expresses the same marker).

Cells expressing the genes are shown on top of the other cells (order = T). <b>(G–I)</b> UMAPs showing only lobula neuropil glia integrated from all stages, displaying the expression levels of <i>CR30009</i>, <i>gcm</i> and <i>E(spl)malpha-BFM</i>. Cells expressing the genes are shown on top of the other cells (order = T). The proposed start for the lobula neuropil ALG (red arrow) and lobula neuropil EG (green arrow) glia are shown in each panel.