Raw Data for the article "Rolling back human pluripotent stem cells to an eight-cell embryo-like stage"

Yet, their spectrum and potency remain less well characterized than in rodents, thwarting application. Particularly, in contrast to their mouse counterparts, human totipotent-like cells corresponding to the time of zygote genome activation (ZGA) or eight-cell (8C) stage have not yet been generated.

Here, we report a quick and controllable, exogenous factor-free, methodology for producing cells transcriptionally and epigenetically similar to 8C embryonic cells from human PSCs. Single-cell multiomics characterization identified key molecular events associated with this conversion, including a partial shutdown of the pluripotency network. Loss-of-function experiments also showed fundamental roles for two factors highly enriched in 8C human embryos, DPPA3/STELLA (a master regulator of DNA methylation in oocytes) and TPRX1 (an ETCHbox family transcription factor lacking in mouse).