Data · dataset · 2023
Nanostring
Listed in Teesside University Research Data Repository
These data are pertinent to our forthcoming publication in Cell Reports.
Description
These are the raw data files from Nanostring analysis of RNA extracted from F6 cells exposed to BMP4+FGF2 for 72 hours, then EGF+FGF2 +/- doxycycline and/or chiron for 48 hours. Samples labelled BG were exposed to EGF+FGF2 alone, samples labelled D were exposed to EGF+FGF2+Dox, samples labelled C were exposed to EGF+FGF2+Chiron, samples labelled DC were exposed to EGF+FGF2+Dox+Chiron.
RNA was extracted from cells, and gDNA eliminated, using the RNeasy Mini Kit (Qiagen, 74104). The Nanostring PanCancer Pathways panel was used. Hybridization, purification and imaging on the nCounter system were conducted in accordance with manufacturers’ protocols.
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Triplicate samples were analysed for each condition. These are the raw data files. For anaysis, raw protein count data were processed by applying background thresholding and content normalization in NanoString nSolver 4.0.
Comparisons were made, by group, to the EGF+FGF2 alone condition.
Links
Where it is published
- DOI doi.org/10.17632/v4k77m3rxm.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Imaging 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/v4k77m3rxm.1 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:imaging | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |