Data · dataset · 2024
An experimental approach in analyzing the cell cycle dynamics of food-entrapping cells of sponges: Leucosolenia
Listed in Teesside University Research Data Repository
Data stored here support the conclusions presented in the publication "An experimental approach in analyzing the cell cycle dynamics of food-entrapping cells of sponges".
Description
Lecucosolenia corallorrhiza (class Calcarea) possesses several types of proliferating cells with food-entrapping cells - choanocytes - serving as the main proliferative population of cells. The less proliferative cell populations are represented by epithelial-like pinacocytes and porocytes.
Using various experimental techniques, we revealed that choanoderm of L. corallorrhiza harbours large population of clowly cycling choanocytes. CLSM Z-stacks are stored in .ims format, represented by the original image as well as spots used to count nuclei/EdU-positive nuclei. Folder "EdU accumulation" contains CLSM Z-stacks of sponges treated with EdU for 2 h and 6 h ("Cell cycle dynamics" experiment).
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Tissues are stained with DAPI (channel 1; cell nuclei), anti-acetylated alpha-tubulin antibodies (channel 2; choanocyte flagella); Sulfo-Cy3 azide + EdU (channel 3; S-phase nuclei). Folder "Growth fraction" contains CLSM Z-stacks of sponges treated with 1 mkg/ml of aphidicolin for 4.5 days followed by 6 h of EdU incubation ("Estimating the growth fraction" experiment). Tissues are stained with DAPI (channel 1; cell nuclei), anti-acetylated alpha-tubulin antibodies (channel 2; choanocyte flagella); Sulfo-Cy3 azide + EdU (channel 3; S-phase nuclei).
Folder "G2-M analysis (colocalization study)" contains CLSM Z-stacks of sponges after EdU treatment of different length ("G2/M-phase analysis" experiment). Tissues are stained with DAPI (channel 1; cell nuclei), anti-acetylated alpha-tubulin antibodies (channel 2; choanocyte flagella); Sulfo-Cy3 azide + EdU (channel 3; S-phase nuclei); anti-Ser10-phosphorylated histone 3 antibodies (channel 4; M-phase nuclei). Folder "Proliferating cell types" contains CLSM Z-stacks of sponges after CellTracker incubation and 6 hours of EdU treatment ("Identifying proliferating cell types" experiment).
Tissues are stained with DAPI (channel 1; cell nuclei), anti-acetylated alpha-tubulin antibodies (channel 2; choanocyte flagella); Sulfo-Cy3 azide + EdU (channel 3; S-phase nuclei); CellTracker Deep Red (channel 4; cytoplasm). Folder "Flow cytometry data" contains raw .csv data obtained during flow cytometry analysis of DNA content in single-cell suspensions after ACME dissocitation of intact tissues. Suspensions are stained by Propidium Iodide (PE-A channel).
Links
Where it is published
- DOI doi.org/10.17632/r3ydrmryzg.2 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science · Zoology
- Inferred from text
- Image 75%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/r3ydrmryzg.2 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].anzsrc:group:3101 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3109 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Zoology'] |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |