Omics · study · 2026
Trans-kingdom, rapid, affordable Purification of RISCs (TraPR) enrichment of small RNAs from Cuscuta campestris in vitro haustoria and flanking host stems of Arabidopsis thaliana
Listed in NCBI GEO
The obligate parasitic plant Cuscuta campestris delivers trans-species microRNAs (miRNAs) into host plants to silence host mRNAs.
Description
Here, the genetic requirements for biogenesis, movement, and function of these miRNAs were investigated. Primary miRNA transcript accumulation precedes mature miRNA accumulation by 24 to 48 hours.
Trans-species miRNAs accumulate in host tissues a short distance from the site of parasite attachment. Trans-species miRNAs require C. campestris but not host Dicer-Like 1 (DCL1) for accumulation. These miRNAs specifically avoid Argonaute (AGO) loading in C. campestris tissue where they instead accumulate as miRNA/miRNA* duplexes.
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After arrival and short-distance spreading in host tissues, they are loaded onto host AGO proteins including AGO1 and AGO2. This study clarifies the transcription, dicing, delivery, and function of C. campestris trans-species miRNAs. We propose that selective avoidance of self AGO-loading is a mechanism to facilitate high rates of delivery of these "export only" miRNAs to host tissues.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333470 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333470 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1471154 ncbi.nlm.nih.gov/bioproject/PRJNA1471154 ↗
project · from NCBI GEO
- PubMed 42417192 pubmed.ncbi.nlm.nih.gov/42417192 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Arabidopsis thaliana · Non-coding RNA profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE333470 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:3702 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |