Sulfotransferase Signaling Sustains Fibroblast Identity and Antagonizes Therapeutic Cardiac Reprogramming

To investigate the role of JUNB in fibroblast fate stability during cardiac reprogramming, we performed CUTandRUN profiling of JUNB binding in mouse embryonic fibroblasts undergoing Mef2c, Gata4, and Tbx5 (MGT)-mediated cardiac reprogramming. JUNB occupancy was examined under control conditions and following perturbation of the fibroblast identity barrier gene Chst7.

These datasets were generated to define genome-wide JUNB chromatin binding patterns and to investigate how extracellular signaling pathways influence JUNB-mediated regulation of chromatin accessibility and transcriptional programs during cell fate conversion. The resulting data provide a resource for studying transcription factor occupancy, chromatin regulation, and mechanisms of cell identity maintenance during direct cardiac reprogramming.