Data with DNA damaged-induced phosphorylation of a replicative DNA helicase results in inhibition of DNA replication through attenuation of helicase function - NAR 2024

The MS2 fragmentation of the phosphorylated and nonphosphorylated T518Q containing peptide is included in sheet 2 of the excel document. Results are also reported from MS facility as a .doc report. Raw data used to create the final figures in the paper in a pptx format is the final inclusion.

Following are brief explanations of all columns in the table of the MS data (peptide list) : Sequence - the peptide sequence identified # PSMs - how many times a spectrum has been matched to this peptide sequence ( a crude quantitative value) # Proteins - how many protein(s) this peptide can be inferred to # Protein groups - similar with above but several proteins can be grouped to one protein group if no unique peptides can be identified to discriminated one from the other Protein group accessions - Uniprot (or other protein database) accession number Modifications - PTMs identified on the peptide deltaCn - the difference of XCorr (search engine score) between the best match and the second best match (which is a match to another peptide, usually only the best match is considered as the "correct" match) phosphoRS site probabilities - Predicted probabilities of PTM localization XCorr - search engine score from Sequest HT, the higher XCorr the more confident the identification Charge, MH+, RT - basic probabilities of the peptide ion deltaM - mass difference between the theoretical and detected peptide ion # Missed cleavages - how many K/Rs left in the peptide sequence, which should be cleaved by trypsin but are not.