Table · dataset · 2026
<p>qPCR CT values of Figs 3–8.</p>
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<div> <p>Background</p><p>Gut epithelium serves as a critical barrier against <i>Trichinella spiralis</i> larval invasion, parasite-derived galectins are involved in host–parasite interactions, including invasion and immune modulation.
Description
This study investigated the role of <i>T. spiralis</i> galectin (Tsgal) in gut epithelial barrier disruption and larval invasion.</p> <p>Methodology/principal findings</p><p>Recombinant Tsgal (rTsgal) was expressed <i>in vitro</i> and its interaction with intestinal epithelial cells (IEC) was ascertained using immunofluorescence test (IFT) and confocal microscopy.
The rTsgal-specific binding IEC proteins were identified by GST pull-down, mass spectrometry (MS) and Co-immunoprecipitation (Co-IP) assay. Intestinal epithelial integrity damage induced by rTsgal was evaluated by IFT, qPCR and Western blotting analysis. The <i>in vitro</i> larva invasive assay was also conducted.
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The results showed that rTsgal bound and interacted specifically with MUC13 receptor in Caco-2 cells. rTsgal activated MUC13-ROCK2/MAPK pathway, reduced the expression of gut epithelial tight junctions (TJs; ZO-1, E-cad, Occludin and Claudin-1), increased gut epithelial permeability, disrupted gut epithelial integrity and barrier functions, thereby facilitated larva invasion of Caco-2 monolayers. Moreover, MUC13 receptor inhibitor (Cuc D), MUC13 knockdown of Caco-2 cells, ROCK2 inhibitor (KD025) and p38-MAPK inhibitor (SB203580) suppressed this pathway activation, restored gut epithelial integrity, and impeded larval invasion.</p> <p>Conclusions</p><p>Tsgal disrupted gut epithelial integrity and mediated larval invasion through binding to MUC13 receptor and activating ROCK2/MAPK pathway.
Tsgal might be a potential target molecule for development of novel drugs and vaccines to block <i>T. spiralis</i> infection.</p></div>
Links
Where it is published
- DOI doi.org/10.1371/journal.pntd.0014684.s004 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
- From keywords
- Astronomy & Astrophysics · Biochemistry and cell biology · Cancer · Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Genetics · Humanities · Immunology · Infectious diseases · Inorganic chemistry · Life Sciences · Mass spectrometry · Medicine & Health · Microbiology · Ocean & Atmospheric Science · Research, science and technology policy · Social Science
- Inferred from text
- Computed tomography 50% · Microscopy 75%
Provenance · 1 source records, 27 field assertions
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