<p>The G protein of low-temperature-sensitive <i>rhabdovirus</i> is palmitoylated.</p>

The palmitoylated MSRV G, VHSV G and IHNV G and the immunoprecipitated VHSV G, IHNV G and MSRV G were blotted by HRP-streptavidin and His mAb, respectively. <b>(G-H)</b> ZF4 cells were transfected with the plasmid encoding VHSV G for 24h at 28 °C and 16 °C. Then, the cells were stimulated with CHX (1 µg/mL) and lysed at the indicated time points (0 h, 1 h, 2 h, and 4 <b>h)</b>. The protein levels of VHSV G in the cell lysate were measured by Western blotting. <b>(I-J)</b> ZF4 cells were transfected with the plasmid encoding IHNV G for 24h at 28 °C and 16 °C. Then, the cells were stimulated with CHX (1 µg/mL) and lysed at the indicated time points (0 h, 1 h, 2 h, and 4 <b>h)</b>.

The protein levels of IHNV G in the cell lysate were measured by Western blotting. <b>(K)</b> ZF4 cells were transfected with the plasmid encoding MSRV G and were then treated with Orlistat (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The MSRV G protein levels were measured by Western blotting. <b>(L)</b> ZF4 cells were transfected with the plasmid encoding MSRV G and were then treated with 2-BP (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The MSRV G protein levels were measured by Western blotting. <b>(M-N)</b> ZF4 cells were transfected with the plasmid encoding VHSV G and IHNV G and were then treated with Orlistat (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The VHSV G and IHNV G protein levels were measured by Western blotting. <b>(O-P)</b> ZF4 cells were transfected with the plasmid encoding VHSV G and IHNV G and were then treated with 2-BP (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The VHSV G and IHNV G protein levels were measured by Western blotting.</p>