Chlamydia serovars in uterus- scRNA-seq, CITE-seq and TCRab [I33]

Mice received 1% transgenic NR23.4 (CD8) and NR1 (CD4) T cells intravenously 24 hours before infection to track antigen-specific CD8 and CD4 T cell responses. Mice were infected transcervically with C. trachomatis serovars D (UW-3/Cx) and L2 (434/Bu) (5x10E6 IFU) at day 0 and rechallenged at day 28 post-infection (5x10E6 IFU).

T cells were isolated from the uterus of C57BL/6J mice at multiple time points: 5 and 15 days post-primary infection and 3 and 5 days post-secondary challenge. All animals were pre-treated with medroxyprogesterone acetate to synchronize estrous cycles.