Data · dataset · 2026
Identification and functional study of a novel antimicrobial peptide, QUB-1976, from the skin secretion of the frog, <i>Phyllomedusa bicolor </i>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32640186.v1
Antibiotic resistance has led to a significant shortage of effective antibiotics.
Description
The aim of this study is to identify a novel peptide drug that has the potential to kill microorganisms and to evaluate its therapeutic value. From the skin secretion of Phyllomedusa bicolor, a precursor cDNA encoding QUB-1976 was obtained through molecular cloning.
Sequence alignment confirmed its classification within the phylloseptin family. I-TASSER predicted it to be an alpha-helical peptide. The mature peptide sequence, FLSLIPKIVSGVAALAKHL-NH2, was synthesised via Fmoc solidphasepeptide synthesis.
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Subsequent reverse-phase high-performance liquid chromatography purification and MALDI-TOF mass spectrometry confirmed successful synthesis. Antimicrobial activities against Staphylococcus aureus NCTC 10788, Escherichia coli NCTC 10418 and Candida albicans ATCC 10231, were evaluated, the Minimum Inhibitory Concentration (MIC) / Minimum Bactericidal Concentration (MBC) of QUB-1976 against S. aureus was 0.25/0.25 μM, against E. coli was 4/8 μM, and against C. albicans, was 16/16 μM. QUB-1976 shows the strongest antibacterial effect against S. aureus, with decreasing effectiveness against E. coli and C. albicans.
QUB-1976 also inhibits non-small cell lung cancer cells with an IC50 of 10.48 μM, indicating potential anticancer activity, and its haemolytic activity with an HC50 of 24.42 μM, suggesting blood toxicity at this concentration.<br><br><i>Thesis is embargoed until 31st December 2029</i>.<br><br>
Links
Where it is published
- DOI doi.org/10.17034/32640186.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences
- Inferred from text
- Cancer 75% · Mass spectrometry 75%
Provenance · 3 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32640186 | 12 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32640186 | 12 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32640186 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |