Data from: Mechanistic dissection of BLTP2 targeting to ER-PM contact sites

We demonstrate that a conserved adapter protein, which we name bilbobaggins (bbo), is required for targeting of Hobbit to ER-PM contacts; importantly, loss of bbo phenocopies loss of hobbit, indicating that bbo is required for hobbit function. Additionally, our structure-function analyses show that cis-acting sequences in the C-terminal tail of Hobbit are also required for ER-PM targeting. Crucially, our data indicate that these cis-acting sequences and Bbo binding are independent and likely sequential mechanisms that we propose function like a “hook” and “latch” to govern Hobbit targeting.

Thus, we define a new regulatory paradigm governing targeting of BLTPs to membrane contact sites.