Omics · study · 2026
Mouse embryoid body differentiation in Profilin-2 3'UTR cis-element mutants
Listed in NCBI GEO
The coordinated actions of post-transcriptional regulation are essential for stem cell differentiation.
Description
The Profilin-2 transcript can be targeted by microRNAs and RNA binding proteins, specifically Ago2 and ESCC family of miRNAs -- miR-291-3p/294-3p/295-3p/302-3p -- and the Iron Regulatory Proteins IRP1/2. Earlier studies described the role of the conserved Profilin-2 ESCC miRNA site.
This study describes the role of the conserved Profilin-2 IRP/iron-response-element site by CRISPR-mediated disruption of the Profilin-2 3'UTR iron response element site. The transcriptomic data reported indicates impaired germ layer formation and distribution during embryoid body differentiation when compared to wild-type mouse embryonic stem cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE288nnn/GSE288017 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE288017 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1215792 ncbi.nlm.nih.gov/bioproject/PRJNA1215792 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE288017 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |