Omics · study · 2026
RNAseq analysis of overexpression of KLK5 in the HGSOC epithelial cells on ovarian cancer
Listed in NCBI GEO
Ovarian cancer remains a significant global health burden, with high-grade serous ovarian cancer (HGSOC) representing the most lethal subtype.
Description
Bulk RNA-seq analysis revealed KLK5 (Kallikrein-5, a serine protease) upregulation in ovarian cancer, correlating with shortened disease-free survival (DFS) and advanced stage in TCGA cohorts. In vitro functional assays further demonstrated that KLK5 promotes metastatic potential in ovarian cancer cells, indicating its role in disease progression.
Single-cell and spatial transcriptomic analysis identified elevated KLK5 expression specifically in HGSOC epithelial cells. Concurrently, KLK5-high tumors exhibited enrichment of collagen-related genes (COL1A1/2, COL6A2) within the fibroblast compartment, suggesting KLK5-driven epithelial-stromal crosstalk mediated by collagen signaling. The co-expression of KLK5 with AGRN implicated extracellular matrix (ECM) remodeling as a potential progression mechanism.
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Immune profiling revealed that KLK5-high tumor microenvironments (TMEs) harbor increased populations of immunosuppressive myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs). Enhanced CD8+ T cell-macrophage interactions observed in these KLK5-high tumors may drive macrophage reprogramming and contribute to immune evasion. In conclusion, KLK5 promotes HGSOC progression through stromal activation, AGRN-mediated ECM alterations, and the reshaping of the TME towards an immunosuppressive state.
KLK5's dual role as both a prognostic biomarker and a potential therapeutic target positions it as a promising candidate for precision oncology in HGSOC.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE315nnn/GSE315531 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE315531 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1398212 ncbi.nlm.nih.gov/bioproject/PRJNA1398212 ↗
project · from NCBI GEO
- PubMed 41866470 pubmed.ncbi.nlm.nih.gov/41866470 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · Disease 75% · RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE315531 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |